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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
01/03/2004 |
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Data da última atualização: |
14/05/2004 |
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Autoria: |
SOUZA, F. A. de; DECLERCK, S. |
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Título: |
Mycelium development and architecture, and spore production of Scutellospora reticulata in monoxenic culture with Ri T-DNA transformed carrot roots. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Mycologia, Bronx, v. 95, n. 6, p. 1004-1012, 2003. |
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Idioma: |
Inglês |
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Conteúdo: |
Mycelium development and architecture and spore production were studied in Scutellospora reticulata from single-spore isolates grown with Ri T- DNA transformed carrot root-organ culture in monoxenic system. Culture establishment, anastomosis occurrence and auxiliary cell development also were examined. Seventy percent of the pregerminated disinfected spores colonized the transformed carrot roots. After 8 mo, the average spore production was 56 (24-130) per 30 cm3 of medium. Of the spores produced, 75% germinated and produced new generations in monoxenic culture. The mycelium net- work was formed by thick light-brown hyphae, which exhibit two major architecture patterns related to either root colonization or resource exploitation, and lower-order hyphae, bearing auxiliary cells, branched absorbing structures (BAS) , hyphal swellings (HS) and forming anastomoses. BAS were formed abundantly in extramatrical mycelium and frequently had HS resembling vesicles, a feature not previously re- ported in the Gigasporaceae, to the best of our knowledge. Few anastomosis were observed within the mycelium and most often corresponded to a healing mechanism that form hypha bridges to reconnect broken hyphae or overcoming obstructed areas within a hypha. Numerous auxiliary cells were produced during culture development and their role was inferred.
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Palavras-Chave: |
Root; Scutellospora reticulata; Vesicular arbuscular mycorrhizal. |
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Thesagro: |
Cenoura; Micorriza Vesicular Arbuscular; Raiz. |
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Thesaurus NAL: |
carrots; roots; vesicular arbuscular mycorrhizae. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02102naa a2200241 a 4500
001 1625777
005 2004-05-14
008 2003 bl --- 0-- u #d
100 1 $aSOUZA, F. A. de
245 $aMycelium development and architecture, and spore production of Scutellospora reticulata in monoxenic culture with Ri T-DNA transformed carrot roots.
260 $c2003
520 $aMycelium development and architecture and spore production were studied in Scutellospora reticulata from single-spore isolates grown with Ri T- DNA transformed carrot root-organ culture in monoxenic system. Culture establishment, anastomosis occurrence and auxiliary cell development also were examined. Seventy percent of the pregerminated disinfected spores colonized the transformed carrot roots. After 8 mo, the average spore production was 56 (24-130) per 30 cm3 of medium. Of the spores produced, 75% germinated and produced new generations in monoxenic culture. The mycelium net- work was formed by thick light-brown hyphae, which exhibit two major architecture patterns related to either root colonization or resource exploitation, and lower-order hyphae, bearing auxiliary cells, branched absorbing structures (BAS) , hyphal swellings (HS) and forming anastomoses. BAS were formed abundantly in extramatrical mycelium and frequently had HS resembling vesicles, a feature not previously re- ported in the Gigasporaceae, to the best of our knowledge. Few anastomosis were observed within the mycelium and most often corresponded to a healing mechanism that form hypha bridges to reconnect broken hyphae or overcoming obstructed areas within a hypha. Numerous auxiliary cells were produced during culture development and their role was inferred.
650 $acarrots
650 $aroots
650 $avesicular arbuscular mycorrhizae
650 $aCenoura
650 $aMicorriza Vesicular Arbuscular
650 $aRaiz
653 $aRoot
653 $aScutellospora reticulata
653 $aVesicular arbuscular mycorrhizal
700 1 $aDECLERCK, S.
773 $tMycologia, Bronx$gv. 95, n. 6, p. 1004-1012, 2003.
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